The present study was an effort to explore the effect of pet interaction on stress and mood among pet-owners and non-owners in collectivistic culture of Pakistan, where empirical investigation of attitude toward animals and the impact of human-animal interaction has been lacking. The study was experimental in nature, with a pretest-posttest between-subject design. Sample of pet-owners (n = 90) and non-owners (n = 90) was taken from University of Veterinary and Animal Sciences, Lahore. Both, male and female participants in the age range of 18 to 26 years were included. A Russian Samoyed mix puppy and a Persian kitten were selected for the interaction. The instruments used were Short Stress State Questionnaire (Helton, 2004), Brief Mood Introspection Scale (Mayer & Gaschke, 1988) and Pet Interaction Observation Checklist (PIOC). Four experimental groups (Group 1 and 2: pet-owners interaction with either dog or cat; Group 3 and 4: non-owners interaction with either dog or cat) and two control groups (for pet-owners/non-owners reading magazine) were present. A pre-assessment of stress and mood was taken with the respective questionnaires. Then, on the basis of random assignment, the participant carried out the assigned activity. Interaction of participant was observed and recorded with PIOC. At the end, post-assessment was taken and debriefing was done. Statistical analysis of data showed a significant negative relationship of stress (worry and distress) with pleasant/unpleasant mood. In addition, reduction in stress and increase in positive mood was found for both pet-owners and non-owners for the pet interaction groups as compared to control groups. However, no significant differences were found between the dog interaction group and cat interaction group for both, pet owners and non-owners. Moreover, pet-owners had lower scores on stress (worry) and higher scores on mood after pet interaction as compared to non-owners. Nonsignificant differences were found between men and women for stress and mood among pet-owners and non-owners. Lastly, it was shown that individuals who interacted with pets for more than five minutes had lower scores on worry and higher scores on pleasant mood as compared to those who interacted for five minutes or less.